I've got a bunch of .ab1 files from sanger sequencing over an exon. Any free programs to quickly find the SNP?
I tried to convert .ab1 to fastq (use Staden package) with the intention to use BWA; but it turns out Staden is an assembly program which gives me one long config from which I cannot call SNP. I guess there could be some commands from Staden that can do the job, but it has a monsterous manual with 700 pages...
So any quick way to call SNP from .ab1 file?