Question: Visualizing Vcf Files With Igv
1
gravatar for GPR
7.3 years ago by
GPR320
Mexico
GPR320 wrote:

Hello, I have generated vcf files using VarScan v2.3.2 and am trying to visualize them in IGV. I have indexed the files with either IGV-tools or tabix, followed by bgzip in both cases. When I upload one of these files in IGV, it appears as if 2 tracks are present (the reference track and the vcf track), but I can only see the transcript reference one. I tried changing the track height and expanding the tracks with no success. Can somebody comment on what's the catch with this? Thanks, G.

vcf igv • 6.3k views
ADD COMMENTlink modified 7.3 years ago by samsara600 • written 7.3 years ago by GPR320

hopefully you mean you ran bgzip and then indexed with tabix

ADD REPLYlink written 7.3 years ago by brentp23k
0
gravatar for samsara
7.3 years ago by
samsara600
The Earth
samsara600 wrote:

You should be able to visualize VCF file in IGV if you have index file for corresponding VCF file. My assumption is, there must be something wrong with yor VCF file.

I have visualized reference genome, BAM file and VCF file that contains SNP called from the same BAM file. When I zoom the view to the fullest and go to the region of one of SNPs ( I looked into my VCF file and picked one SNP location) I can clearly see my SNP of interest.

ADD COMMENTlink written 7.3 years ago by samsara600
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