Expression Studies Of Alternative/Splice Variants Of A Gene
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11.5 years ago
jvknp ▴ 10

hi is there any sophisticated method (other than Real Time PCR) to study the expression of splice variants of a gene which differ by one or two codons

mrna • 2.9k views
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On StackOverflow this question would be closed as too open and vague. Suggest you improve it to avoid the same fate.

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Elobarate your question...

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I was studying the relative abundance of two splice variants (mRNA) under different stress conditions by qPCR. These two splice variants differ by one codon (revealed by RNA-seq data).

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11.5 years ago

I'm not sure what you are really asking ("sophisticated method" is a bit vague), but there are several approaches to study changes in splicing using RNA-seq data, such as:

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