Question: Expression Studies Of Alternative/Splice Variants Of A Gene
1
gravatar for jvknp
6.5 years ago by
jvknp10
jvknp10 wrote:

hi is there any sophisticated method (other than Real Time PCR) to study the expression of splice variants of a gene which differ by one or two codons

mrna • 1.8k views
ADD COMMENTlink modified 5.7 years ago by Biostar ♦♦ 20 • written 6.5 years ago by jvknp10

On StackOverflow this question would be closed as too open and vague. Suggest you improve it to avoid the same fate.

ADD REPLYlink written 6.5 years ago by Neilfws48k

Elobarate your question...

ADD REPLYlink written 6.5 years ago by aravind ramesh510

I was studying the relative abundance of two splice variants (mRNA) under different stress conditions by qPCR. These two splice variants differ by one codon (revealed by RNA-seq data).

ADD REPLYlink written 6.5 years ago by jvknp10
2
gravatar for Steve Lianoglou
6.5 years ago by
Steve Lianoglou5.0k
US
Steve Lianoglou5.0k wrote:

I'm not sure what you are really asking ("sophisticated method" is a bit vague), but there are several approaches to study changes in splicing using RNA-seq data, such as:

ADD COMMENTlink modified 6.5 years ago • written 6.5 years ago by Steve Lianoglou5.0k
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