Multiple Sequence Alignment Score
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10.6 years ago
Lee Katz ★ 3.1k

Does anyone have a script to determine the score of a multiple sequence alignment? Hopefully using BioPerl?

multiple bioperl scoring scripting • 7.1k views
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I almost thought that someone had asked this until I went into the question, but it looks like it hasn't come up yet. Pariwise Local And Global Alignment

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10.6 years ago
Lee Katz ★ 3.1k

Per Alastair's comment, I tried MstatX, at https://github.com/gcollet/MstatX.

The program is easy to use and has several ways of calculating the score. However, it bothers me a little that it doesn't output a final score to the termal. It prints a score for each column into a file, which I was able to sum up. It also bothers me that it doesn't come packaged with a simple matrix for DNA and so it is only optimized for protein. I quickly made up a DNA matrix on the spot which may not be technically correct.

tar zxvf gcollet-MstatX-31481c6.tar.gz
cd gcollet-MstatX-31481c6
make
./mstatx -ma path/to/file -b -sp data/dna.mat
perl -e 'while(<>){$score+=$_;}print "score\n";' < file.cons  The file I made (probably isn't the most correct thing I could have made) H DNA matrix D DNA matrix by Lee Katz R LIT:1902106 PMID:1438297 A Henikoff, S. and Henikoff, J.G. T Amino acid substitution matrices from protein blocks J Proc. Natl. Acad. Sci. USA 89, 10915-10919 (1992) * matrix in 1/3 Bit Units M rows = ATCGN-, cols = ATCGN- 2. -1. 2. -1. -1. 2. -1. -1. -1. 2. -1. -1. -1. -1. -2. -2. -2. -2. -2. -2. -2. //  ADD COMMENT 1 Entering edit mode I also tried alistat from the squid package, which does not give a score ADD REPLY 1 Entering edit mode MstatX now give a global score of an alignment = the sum of column scores divided by the number of columns. ADD REPLY 0 Entering edit mode 9.5 years ago muscle3.8 has a 'spscore' option which computes an SP objective score for a multiple sequence alignment. e.g. path/to/muscle -spscore file_name e.g. to extract just the score into a variable (psuedocode): Compute SP score with muscle (e.g. path/to/muscle -spscore file_name -log <log_file>) Read log file Iterate through each line of file If line contains string 'SP=' (perl e.g. /SP=/) match 'SP=' (perl e.g. =~ /SP=/) Print segment after match (perl e.g. print')


NB: You could even extract the matching line with unix 'grep' Download muscle from: http://www.drive5.com/muscle/

"The Father. The Son. The Holy Spirit. And the South African National Bioinformatics Institute."

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