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Question: Expressed Sequences Tags For Identifying Mirna Promoters
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gravatar for ancient_learner
5.9 years ago by
ancient_learner • 610
India
ancient_learner • 610 wrote:

Can anybody tell me how ESTs can be used for identifying mirna promoters? I have gone through few papers like http://www.pnas.org/content/104/45/17719.long and http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241639/ but couldn't actually get the proper details.

est mirna • 1.1k views
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modified 5.9 years ago by 2011101101 • 100 • written 5.9 years ago by ancient_learner • 610

Are you asking how to find sequences on the genome that regulate miRNA transcription using ESTs?

ADD REPLY • link written 5.9 years ago by jgibbons1 • 50

ya i mean that only:promoters

ADD REPLY • link written 5.9 years ago by ancient_learner • 610
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gravatar for jgibbons1
5.9 years ago by
jgibbons1 • 50
jgibbons1 • 50 wrote:

Assuming you've already identified probable miRNA from the ESTs you could map the miRNA back to the genome and look for promoters that are known to regulate nearby genes.
ADD COMMENT • link written 5.9 years ago by jgibbons1 • 50
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gravatar for 2011101101
5.9 years ago by
2011101101 • 100
2011101101 • 100 wrote:

there is another paper Characterization and Identification of MicroRNA Core Promoters in Four Model Species perhaps it's help you
ADD COMMENT • link written 5.9 years ago by 2011101101 • 100
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