I have a question concerning the gene ontology tool DAVID: In DAVID it is possbile to create a sublist after a first round of functional annotation. The sublist is created by the user himself, i.e. if I check the tickbox for the GO-Term Extracellular Matrix, I can create a sublist with that term (or several terms selected by that way). My term has p< 0.05 but corrected p-value (Benjamini) of 0.1 (not significant). If I choose now my sublist and perform a second round of analysis (background keeps the same) I get significant values (lets say p<0.000005 and Benjamini p<0.0001). In a third round of analysis by creating a 3rd sublist, with the same (!) terms, the values do not change! Do you think that this is correct? Can one perform that way of analysis? Is there a justification/explanaition for this?
Thank you very much,