Question: Count Vs Dispersion Plot
gravatar for Eric
6.7 years ago by
Eric90 wrote:

I am analyzing my RNA-seq data with cummeRbund. I have four conditions, 5 biological replicates per condition. Why do the count vs dispersion plots look more or less identical for all four conditions? I can see from the cummeRbund vignette that this is supposed to be the case; I just don't know why.

Any insights would be appreciated.


ADD COMMENTlink modified 6.7 years ago by Sean Davis25k • written 6.7 years ago by Eric90
gravatar for Sean Davis
6.7 years ago by
Sean Davis25k
National Institutes of Health, Bethesda, MD
Sean Davis25k wrote:

The count-vs-dispersion relationship is roughly governed by the biological counting process used to quantify RNA-seq data. You might take a look at papers by Simon Anders and Wolfgang Huber (authors of DEXSeq and DESeq) for some background and details.

ADD COMMENTlink written 6.7 years ago by Sean Davis25k
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