Using the 2-delta delta Ct method I have determined the relative gene expression in a panel of 7 treated and untreated cell lines-part of data shown below (C=untreated; T=treated):
Sample RQ RQ RQ D556C 2.6588333 2.1409998 4.9944763 D556T 9.368949 7.3571334 18.470724 D384C 14.2748 10.199899 18.23931 D384T 11.659178 9.1894222 7.546831
I used Prism to represent the data as a bar chart with error bars
For each cell line I want to look at the fold change in expression between the untreated and treated, and so for each replicate and cell line I normalised the treated to the untreated-part of data shown below:
Sample Foldchange Foldchange Foldchange D556C 1 1 1 D556T 3.5 3.4 3.7
Using prism when I graphically represent the fold change data I get error bars for the treated cell line, how do I get error bars for the untreated cell line to which the treated has been normalised? i.e all untreated replicates have a value of 1. When I look in the literature results are displayed with error bars on the untreated 1 x sample?