Question: Qpcr Ratios Problem
1
gravatar for ThePresident
7.5 years ago by
ThePresident60
Canada
ThePresident60 wrote:

Hello,

I have a probably (very) simple question that I can't quit figure out.

I've performed some qPCR on DNA in order to calculate ratios of two different DNA configurations. In some conditions, configuration A is higher, in others it's configuration B that is higher. I also measured a signal for an internal gene that never changes (kind of HKG). Now, I would like to calculate % of A. How do you do that?! I can use delta-delta-Ct method to calculate FoldChange of one configuration relative to the other one. But it would lay better on a graph if I had a percentage of A on B.

Any hints?

Thanks in advance!

• 1.4k views
ADD COMMENTlink modified 7.5 years ago by Devon Ryan97k • written 7.5 years ago by ThePresident60
1
gravatar for Devon Ryan
7.5 years ago by
Devon Ryan97k
Freiburg, Germany
Devon Ryan97k wrote:

You're already familiar with the delta-delta-Ct method, so just use that and multiply by 100.

ADD COMMENTlink written 7.5 years ago by Devon Ryan97k
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