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Question: How Illumina Methylation Array Find Out Whether It Is A Tg In Original Sample
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gravatar for ruansun1983
7.7 years ago by
ruansun1983 • 30
United States
ruansun1983 • 30 wrote:

Illumina methylation 450k detect methylation by converting CG -> TG through bisulfite treatment. Then it detects the signal strength of CG (methylated intensity) versus TG (unmethylated intensity) But what if the site is originally TG? I mean TG without bisulfite treatment.
methylation • 1.5k views
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modified 7.7 years ago • written 7.7 years ago by ruansun1983 • 30
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gravatar for Neilfws
7.7 years ago by
Neilfws ♦ 49k
Sydney, Australia
Neilfws ♦ 49k wrote:

Short answer: the probes on the Illumina 450K platform are designed to examine CpG sites. TG sites are of no interest, since they cannot be methylated.

You should read the details of how the platform was designed - starting point.
ADD COMMENT • link modified 7.7 years ago • written 7.7 years ago by Neilfws ♦ 49k

You didn't quite understand my question. I mean how can they make sure the site is CpG, not TpG in specific sample?

ADD REPLY • link written 7.7 years ago by ruansun1983 • 30

You don't quite understand my answer. They are sure the site is CpG because the probes on the 450K platform are designed to target CpG sites, not TpG sites.

ADD REPLY • link written 7.7 years ago by Neilfws ♦ 49k
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