Question: Purpose Of Y-Shaped Adapters In Illumina Sequencing?
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gravatar for gaelgarcia05
7.2 years ago by
gaelgarcia05210
UK
gaelgarcia05210 wrote:

Hi all,

Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library.

The arms of the Y are unique, and the middle part, connected to the DNA fragment, is complementary.

What are the advantages of this?

My take of this over having fully-complementary adapters (ADAPTER1 - - - - - ADAPTER1) is that:

-Upon primer annealing, the primer in turn would bind to the start AND end of the DNA fragment, and thus not allow synthesis to continue to until the end. Therefore, the next primer would not have a template to bind at the start of the fragment...

Y-shaped adapters also allow for paired-end sequencing. Fragments have a unique sequence on either end, which allows for the first "run" to sequence one side of all molecules, then synthesize the reverse and sequence that.

Am I on the right track?

ADD COMMENTlink written 7.2 years ago by gaelgarcia05210

This is not really a bioinformatics question. It has more to do with protocols in molecular biology.

ADD REPLYlink written 7.2 years ago by Neilfws48k
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