Looking For A Tool To Perform De Novo Assembly On Unassigned Reads From An Illumina Fastq (Reference Guided Assembly Bin)
1
0
Entering edit mode
9.6 years ago

I am having trouble finding a program that will preform a de novo assembly on unassigned reads from an illumina fastq. Are there any good programs that preform this function or have a output of unassigned reads that can be pipelined

assembly • 3.2k views
ADD COMMENT
0
Entering edit mode
8.8 years ago

Hi,

You can begin with Velvet or SOAPdenovo2 that perform de novo assembly on Illumina read sequences.

I would try to assemble all reads (assigned together with not assigned one) so you should obtain contigs from both origin. Similarity search on the resulting contigs could be helpful to identify this origin.

ADD COMMENT

Login before adding your answer.

Traffic: 1255 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6