Question: What Are The Main Uses Of A Consensus Sequence?
1
gravatar for Sheila
7.2 years ago by
Sheila320
United States
Sheila320 wrote:

Forgive me for this question, but could anyone explain to me what are the main uses for a consensus sequence? In other words, what kind of research requires the use of consensus sequences?

If you have VCF files do you need the consensus sequence?

ngs • 3.4k views
ADD COMMENTlink modified 7.2 years ago by lh332k • written 7.2 years ago by Sheila320
1
gravatar for lh3
7.2 years ago by
lh332k
United States
lh332k wrote:

In the context of NGS analysis, consensus sequences provide regions where we can make confident calls, which is missing from variant-only VCFs. They are mostly useful for popgen. For example, you can compute heterozygosity from a consensus sequence, but you cannot do the same with a variant-only VCF.

ADD COMMENTlink modified 7.2 years ago • written 7.2 years ago by lh332k
0
gravatar for Jordan
7.2 years ago by
Jordan1.1k
Pittsburgh
Jordan1.1k wrote:

I think consensus sequence represents which amino acid or nucleotide appears the most frequent in a position. I have seen it used most during estimating motifs and also after multiple alignment. And most of the splice sites are represented by consensus sequences too.

A typical consensus sequence looks like this:

AG[TC]T[CG]NSA

And I'm not sure about the vcf files question. It depends on the kind of analysis you are doing and why you need it I guess.

ADD COMMENTlink modified 7.2 years ago • written 7.2 years ago by Jordan1.1k

So if you have the bam (assembly) files, do you need (or want) the consensus sequence files?

ADD REPLYlink written 7.2 years ago by Sheila320

I never needed the consensus files. Which analysis are you working on? Why do you think you need consensus files?

ADD REPLYlink written 7.2 years ago by Jordan1.1k
0
gravatar for Alex Reynolds
7.2 years ago by
Alex Reynolds30k
Seattle, WA USA
Alex Reynolds30k wrote:

A motif usually has a consensus sequence with high information content at most bases. One could use a consensus sequence to demonstrate that a set of genomic regions (obtained via lab or computational methods) constitutes a particular motif.

ADD COMMENTlink written 7.2 years ago by Alex Reynolds30k
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