Closed:Illumina Miseq Phix Quality Control (Sequencing Error Rates)
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10.9 years ago
anin.gregory ▴ 110

I recently got back a lot of sequencing data from an Illumina MiSeq run with very low genetic variation. I want to make sure that the low genetic variation is a result of natural genetic variation instead of sequencing error. I emailed my sequencing facility and no on has responded. I know that PhiX is commonly used as a quality control to test sequencing error rates.

Does anyone know the sequencing error rate with PhiX on a Illumina MiSeq (NOT HiSeq)??

Thanks!

illumina miseq • 3.9k views
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