Question: Which Strand Of The Mrna Molecule Does The Sequencer Output As A "Read"?
In Illumina Stranded RNA-Seq (using the dUTP method), do the final reads in the fastq files correspond to the initial molecule (that was transcribed), or to the reverse complement of the molecule?
modified 6.0 years ago
6.0 years ago by
gaelgarcia05 • 190