This question may be very simple and basic, but I just need to confirm that I understand the differences among those terminologies in the RNA-Seq context. Suppose I have a sample called SLR, and it is sequenced on 5 lanes, so I have (among other output files) BAM files like L1_SLR, L2_SLR, L3_SLR, L5_SLR and L7_SLR.bam. Here, the letter "L" denotes "lanes". In a typical RNA-Seq experiment, we should expect that reads from those 5 lanes have little variations, right? I am confused with the term "technical replicates" and the "lanes" here -- they're not the same, right? Also, I think these 5 lanes constitute a single "library", that is, one sample corresponds to one library. Am I right? That confused me because I once saw that each library corresponds to a technical replicate (e.g. mutant condition sample having 3 technical replicates, so it has 3 libraries; wild condition sample having 3 technical replicates, so a total of 6 libraries for the expriment). Thank you so much for your clarifications!
A technical replicate is a single sample that is sequence again, Illumina's machines have 8 lanes to produce sequences, but each lane can hold more than one sample if required. Check this simple explanation here http://euler.bc.edu/marthlab/scotty/help.html