Question: How To Validate A Translocation Has Happened By Computing
0
gravatar for siyu
5.8 years ago by
siyu130
China
siyu130 wrote:

Recently, I am calling translocations from the whole genome sequencing data by using DELLY. But it reports many results, so How to validate a translocation has happened by computing. Does anyone have a good idea?thanks !

• 1.3k views
ADD COMMENTlink modified 5.8 years ago by Michael Dondrup45k • written 5.8 years ago by siyu130
1
gravatar for Alex Paciorkowski
5.8 years ago by
Rochester, NY USA
Alex Paciorkowski3.3k wrote:

If you want a second computational method for this, BreakDancer calls structural variations like translocations. But depending what you want to do with this knowledge, at some point you probably should prove it with a karyotype and some FISH probes.

ADD COMMENTlink written 5.8 years ago by Alex Paciorkowski3.3k
1
gravatar for Mikael Huss
5.8 years ago by
Mikael Huss4.6k
Stockholm
Mikael Huss4.6k wrote:

Perhaps you could do a de novo assembly and see which of your predicted translocations are present in the assembly.

ADD COMMENTlink written 5.8 years ago by Mikael Huss4.6k
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