Question: How To Validate A Translocation Has Happened By Computing
gravatar for siyu
7.1 years ago by
siyu130 wrote:

Recently, I am calling translocations from the whole genome sequencing data by using DELLY. But it reports many results, so How to validate a translocation has happened by computing. Does anyone have a good idea?thanks !

ADD COMMENTlink modified 7.1 years ago by Michael Dondrup47k • written 7.1 years ago by siyu130
gravatar for Alex Paciorkowski
7.1 years ago by
Rochester, NY USA
Alex Paciorkowski3.4k wrote:

If you want a second computational method for this, BreakDancer calls structural variations like translocations. But depending what you want to do with this knowledge, at some point you probably should prove it with a karyotype and some FISH probes.

ADD COMMENTlink written 7.1 years ago by Alex Paciorkowski3.4k
gravatar for Mikael Huss
7.1 years ago by
Mikael Huss4.7k
Mikael Huss4.7k wrote:

Perhaps you could do a de novo assembly and see which of your predicted translocations are present in the assembly.

ADD COMMENTlink written 7.1 years ago by Mikael Huss4.7k
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