I want to Arficially simulate the 16s rRNA. I got the abundance file from one of the scientific journal. Now I want to simulate the data and I tried it with Grinder its working fine when I'm running without the primers but once I'm adding the primers its showing the error that some particular sequence doesn't exist. But the sequences are there in the abundance file as well as the reference file. For Primers I have used the same Primers used in paper to amplify the region. iI dont know where I'm going wrong but is someone has done the simulation please let me know how I can it be sorted.
Thanks in advance