5.5 years ago by
The University of Edinburgh, UK
I have had experience with many genome centres in Europe/USA/China. All have had turn around time issues, or serious communication issues at some point and I do not want to post a lasting recommendation or criticism for fear that it would no longer be relevant.
Some advice though:
- pay for the provider to make libraries as these generally come with a data quality guarantee.
- make sure you know who is responsible for dealing with rRNA depletion in your samples (you or the company)
- make sure you are fully aware of what data you get back and what processing has been done to that data. Companies such as BGI that use proprietary linkers/adapters may remove them before giving you the raw data to protect their IP. If this is done aggressively, e.g. returning only that which maps to the genome, you may miss interesting data such as 3' RNA editing events.
- do your homework for what sequencing depth you need in your samples
Basically when ordering make sure the provider is contractually obliged to provide all the things that are important to you