Length Of The Vdj Segment In B Cells
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9.6 years ago

Hi guys,

I'm not sure whether this immunology question can get an answer here, but I was wondering what the length of the VDJ segment is in B cells? Is there any way to find this out bioinformatically?

Thanks a lot!

Cheers, Eric

recombination • 4.3k views
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Your question is a little abridged, I cannot even say if its off-topic or not ;) What is a VDJ segment? Most computational people might not know, please explain your acronyms: see How to ask Good Questions on Technical and Scientific Forums

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Yes, or the asker can link to further information using markdown tags. [Like this]biostars.org)

A VDJ segment looks like it has to do with some random gene in every immune cell.

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Dr. Tipton mentioned some bioinformatics solutions to your question in his Nature immunology paper. You can find the pipeline like this way: enter image description here

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9.6 years ago
Noel ▴ 90

You will find all you need to know here (Not sure you meant length of V(D)J before or after recombination)

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13 days ago
mizraelson ▴ 60

The length of the VDJ region depends on the :

  • V and J genes as they have different length
  • Number of added nucleotides in CDR3 region
  • Heavy chain has D segment while Light doesnt

Usually the average CDR3 receptor length is calculated for the repertoire: https://docs.milaboratories.com/mixcr/reference/mixcr-postanalysis/#cdr3-metrics

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