Question: Merging Illumina Paired End Reads
gravatar for vijay
7.2 years ago by
vijay1.6k wrote:

Dear All,

I have fastq a dataset containing forward and reverse sequences obtained through paired end module of Illumina platform. I am trying to merge these paired end reads. I have a query which I would wish to get cleared before I proceed further. Do I need to get the reverse complement of the reverse sequence dataset in order to carry on with the paired end merging?

I have referred few papers and tutorials on this , but they have not mentioned anything about doing a reverse complement. I am bit confused in this step. Kindly help me out!!!

Responses are highly appreciated!!!

illumina paired-end merge • 8.0k views
ADD COMMENTlink modified 2.3 years ago by Biostar ♦♦ 20 • written 7.2 years ago by vijay1.6k
gravatar for Pierre Lindenbaum
7.2 years ago by
France/Nantes/Institut du Thorax - INSERM UMR1087
Pierre Lindenbaum130k wrote:

duplicate of:

merge paired-end reads

ADD COMMENTlink written 7.2 years ago by Pierre Lindenbaum130k
gravatar for Buttonwood
7.2 years ago by
Buttonwood40 wrote:

Do you mean overlap merge? Both of the reads are from 5'-->3'.

ADD COMMENTlink written 7.2 years ago by Buttonwood40
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