I'm trying to make a nucleotide database for my sequences. After opening
C:Program Files\NCBI\blast-2.2.28+> in the command line, I typed in
makeblastdb -in test_gene.fsa -parse_seqids -dbtype nucl
and I got the error message
Building a new db, current time: 07/11/2013 16:07:43 New DB name: test_gene New DB title: Test_gene Sequence type: Nucleotide Keep Linkouts: T Keep MBits: T Maximum file size: 1000000000B BLAST options error: File test_gene does not exist
Then I tried adding the pathway to find the file
blast-2.2.28+>makeblastdb -in C:\Program_Files\NCBI\blast-2.2.28+\test_gene.fsa -parse_seqids -dbtype nucl and I got the same type of error as before. I'm new to this, sorry if this is obvious to fix.