I am planing to do some mutagensis and need to change the amino acid at specific position for a given gene.
I am now doing manually by counting the CDS sequence position and then confirm with the protein sequence.
I am asking whether there is any easier way to locate the corresponding codon sequence for a give position amino acid quickly.
In NCBI CCDS, I can visualize the codon sequence when I hover the mouse over an amino acid, but I coundn't see the postion, like this https://www.ncbi.nlm.nih.gov/CCDS/CcdsBrowse.cgi?REQUEST=ALLFIELDS&DATA=11118.1&ORGANISM=0&BUILDS=CURRENTBUILDS
Thank you very much in advance,