We are getting RNAseq data (mouse genome) soon. It was done in 2 different cell types and we have to compare and identify the differences between those two transcriptomes. In the tophat2 website the index file for mouse itself shows 14Gb. If we get >25M reads (including replicates) for each cell type how much memory i can request our common server facility? (http://cmsd.uohyd.ernet.in/) I have checked other posts as well but couldn't find the exact answer that suits.
Question: Memory Requirements For Running Bowtie2 Tophat2 And Cufflinks
6.1 years ago by
ancient_learner • 610
ancient_learner • 610 wrote:
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