Question: Using Homer Programs For Denovo Motif : Different Results Among Its Own Versions
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5.7 years ago by
RT10 wrote:

Hi Everyone,

I am trying my first hands-on with denovo motif discovery using HOMER. My sequence file has about 9K sequences that are 30bp each. I used two versions of the Homer programs for denovo motif finding and

-bash-3.2$ input.bed hg19 homer_output -bg background.bed -size given

-bash-3.2$ input.fasta fasta homer_output1 -fasta background.fasta

Firstly, number of results and reported match to the denovo motifs vary between both versions.!

I also used the " homer2 denovo " command and observed that the results are relatively consistent with only. I suspect that, when I give BED file, Homer fetches extra bp upstream and downstream from specified coordinate and So, I am getting a huge number of denovo motifs than the other two.

Is there any reason why the extra bp are required or significant in Homer? or Is there a way to make Homer only fetch the length I want using the BED file?

PS : I also used the - size option, but I don see any difference. From Homer manual -size option : if you wish to find motifs using your peaks using their exact sizes, use the option "-size given" However, for Transcription Factor peaks, most of the motifs are found +/- 50-75 bp from the peak center, making it better to use a fixed size rather than depend on your peak size.

Is there anything I am missing?I would appreciate any help with this.

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