Why Does The Small Rna Sequencing Have Not Duplicates?

Question

[2011101101]

Hi, Rcently,I have read many papers about small rna of plant,smRNA-seq is a mainly method ,but not like RNA-seq that have three duplicates,the small rna-seq not.why???

[Devon Ryan]

It's somewhat difficult to parse your post. Is the following a restatement of your question?

"I've read a number of papers recently focused on small RNA profiling in plants using RNAseq. Unlike regular RNAseq experiments, these seem to lack biological replicates. Does anyone know why that might be?"

If so, can you post links to example papers? At the end of the day, the utility of replicates is dependent upon what you're trying to do. If you're just trying to get an idea of what sort of small RNAs are expressed (i.e., no statistical comparisons between groups) or something like that, then replicates might not be needed.