Question: Mirna Structure: Searching And Scoring
gravatar for Alastair Kerr
8.9 years ago by
Alastair Kerr5.2k
Manchester/UK/Cancer Biomarker Centre at CRUK-MI
Alastair Kerr5.2k wrote:

I wish to compare miRNAs based on their predicted secondary (or tertiary) structure and the sequence of the terminal loop. For this I need to implement a system which can:

  1. identify related miRNAs
  2. align them
  3. fold them
  4. determine the conservation of the sequence of the terminal loop
  5. create a metric that describes the comparison of the two structures

Searching for programs I have found but not yet tested infernal and RSearch the latter looks to be extremely computationally expensive. I am also aware of mfold/unafold.

Any other programs out there? Any heuristics that you can recommend?

homology mirna scoring secondary • 1.6k views
ADD COMMENTlink written 8.9 years ago by Alastair Kerr5.2k
gravatar for User 3869
8.9 years ago by
User 3869100
User 3869100 wrote:

What you want to do seems very similar to miRBase. I thought you could read the following paper before getting start.

The microRNA Registry. Griffiths-Jones S. NAR 2004 32(Database Issue):D109-D111

Anyway, you could try ViennaRNA package which is very popular. I applied it on all sequences of miRBase, and it only costed no more than 20 minutes.

ADD COMMENTlink written 8.9 years ago by User 3869100
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