Suggest Pirna Ngs Small Rna Analysis Pipelines/Workflows
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8.1 years ago
Rm 8.1k

I have small RNA sequencing reads generated using NEB kit. I have tried proTRAC workflow, but not much success.

I need your suggestions for the alternate tools or analysis workflows to detect and analyze piRNAs from NGS data.

workflow • 3.5k views
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7.3 years ago
weiwangumms ▴ 10

please try piPipes. https://github.com/bowhan/piPipes

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concerning piPipes: can i use also the RNAseq pipeline (transcriptome) with single-end reads?

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5.5 years ago
opplatek ▴ 150

You can also try Kraken which has a specific part of pipeline just for piRNA analysis (see examples) http://www.ebi.ac.uk/research/enright/software/kraken

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