I need to remove mids and mid sort my sff files while retaining its sff format.
I intend to used SWAP454 for SNP calling in my sff file.
I am unable to find a proper way of doing s using sfffile program over command line.
sfffile -o roche454_trimmed.sff -e mid.lst -nmft sff/roche454.sff
mid.lst is my MID file is GSMID format.
roche454.sff is my sff file that I need should be trimmed off at MIDs.
Can anyone please suggest?