Question

Limma For Rna-Seq Analysis

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10.4 years ago

narges ▴ 210

Hi,

I want to use Limma for RNA-seq analysis. Its manual in Bioconductor includes a section for RNA-seq case of study but the main paper I can find regarding the limma is for year 2004 which is explaining limma only for microarray. Does any one knows if there is a more recently publication or any other sources which explains limma's algorithm for RNA-seq.

Thank you in advance

limma rna-seq • 5.4k views

ADD COMMENT • link updated 10.4 years ago by Devon Ryan 104k • written 10.4 years ago by narges ▴ 210

score 2 · Answer 1 · 2013-11-12

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10.4 years ago

Devon Ryan 104k

It may not have been published yet, but the preprint is available here.

ADD COMMENT • link 10.4 years ago by Devon Ryan 104k

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Actually I found this unpublished one. I did not go through it but from the title I expected it is regarding the "voom" which I think is a method of normalization but not testing for differential expression. Is it correct or it tests the DE as well?

ADD REPLY • link 10.4 years ago by narges ▴ 210

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Voom transforms things for use in limma, which is what performs the DE.

ADD REPLY • link 10.4 years ago by Devon Ryan 104k