Is it possible to query UCSC using mysql to ask what the resulting amplicon would be, sequence, seq length, chr start & stop positions, GC content etc. Similar to what you get when you manually use the In-Silico PCR function on the website.
Ideally I want to supply a file like this (these are dummy sequences)
tcctaacactggccggctcaatggag F1 accaaccagataacaggaag R1 tccacctctgatctgcaaagtgg F2 gctgactttaaaatctgacacca R2
And get an output with all the data.