Question: What Agilent Interval Files (.Bed) Should I Use For Exome Variant Calling With Gatk?
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gravatar for newDNASeqer
5.0 years ago by
newDNASeqer620
United States
newDNASeqer620 wrote:

I've just read Human Exome Capture Library Coordinates Download on Biostars, but I'm still unsure what intervals from Agilent I should use for my human exome variant calling with GATK. The exome enrichment kit our lab uses is Agilent Exome Capture Kit Sure Select XT Target Enrichment System for Illumina Paired –End Sequencing Library. Version XTV4.

I was able to find a list of different designs on the Agilent website eArray https://earray.chem.agilent.com. I am assuming this design named "SureSelect Human All Exon V4" is for the kit we are using, there're a few .bed files in this design such as "regions, alltracks, covered, etc". Should I use the one named "covered" for my variant calling?

ps: my reference genome is human hg19

Sorry for the naive questions, please be gentle. thanks for reply.

agilent bed gatk • 4.9k views
ADD COMMENTlink modified 5.0 years ago • written 5.0 years ago by newDNASeqer620

Why cant we use the default interval list as mentioned here here

ADD REPLYlink written 14 months ago by Karma200
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