I Need Help Understanding What The '-Max-Frag-Count-Draws' Option Means In Cuffdiff
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10.4 years ago

Dear friends,

Can you explain this complex explanation to me in simple language!!

--max-frag-count-draws <int> Cuffdiff will make this many draws from each transcript's predicted negative binomial random numbder generator. Each draw is a number of fragments that will be probabilistically assigned to the transcripts in the transcriptome. Used to estimate the variance-covariance matrix on assigned fragment counts. Default: 100.

--max-frag-assign-draws <int> For each fragment drawn from a transcript, Cuffdiff will assign it this many times (probabilistically), thus estimating the assignment uncertainty for each transcript. Used to estimate the variance-covariance matrix on assigned fragment counts. Default: 50.

ngs cufflinks differential-expression • 1.9k views
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