Rankprodit Webserver: Maximum Allowable Missing Values

Question

[The]

Hi all,

I'm using the RankProdit server for a rank product analysis for my protein expression. I however stumbled at the following instruction

"Missing data can be represented by NA, na, NaN or nan. Note: Prior to analysis, it is recommended that genes without values for at least two biological replicates for the conditions being tested be filtered out, as no statistics for those genes can be calculated"

I'm not sure what it means. Does that mean I can have only One missing value for a gene or does it mean there must at least two non-NA values for a gene ?

Thanks

Answer 1

[Istvan Albert]

What it says is that if you have only one value for a gene it cannot compute a statistics (mean, variance etc) for it. Therefore you need to remove genes that have fewer than two measurements.

[The]

Thanks Istvan for the reply