Hi everyone, I want to check whether proteins are contained in assembled contigs or not ?
For this After De-novo assembly I made a local data base of contig file, which are in fasta formate :
makeblastdb -in contigsH131.fasta -dbtype nucl -out ecolidb.db
In next step i want i tried tblastn and getting some error message like:
./tblastn -num_threads 2 -comp_based_stats F -query refence.fasta -db ecoli.db -out report.bls
BLAST Database error: No alias or index file found for nucleotide database [ecoli.db] in search path [/home/hiren/Desktop/test/ncbi-blast-2.2.28+/bin::
Can anyone please help me out.
Thank you so much.