Can You Point Me To Slides And (Downloadable) Movies About Critical Aspects Of Affymetrix Technology?

Question

[Chris Evelo]

I have to give a basic presentation about Affymetrix technology as part of a course in Sao Paolo.

I usually focus more on the actual analysis but in this case I need to show how the technology affects the analysis that needs to be done.

Things that come to mind are:

• the Lithographic production technology (I found some nice movies but they are on Youtube)
• the labeling and amplification methods (which are not always linear and thus need to be taken into account)
• the distribution of the probesets over the target sequence (which can be quite different for different probesets and array generations)
• the influence of CG content (which I can probably show best from an actual image with high CG lines, but people might have nicer ways to show that)
• the use of alternative (custom) cdf's for annotation and the influence that has on probeset size
• the relation between memorability and expression (classic present call distribution can show this nicely)
• other things that I did not think about

I am looking for slides/images and maybe movies that would introduce these things nicely for people that do not have a lot of experience using Affymetrix technology.

I did already find some things in Google images, slideshare and figshare. Also found some movies (especially about lithophotographic production) but none that I could easily download without breaching copyright.

Update: this worked perfectly

I have created two presentations. The first was mainly based on slides I received from Chao Quang-Lai from Tufts (through Larry Parnell) while the second about the newer Exons and ST-Gene arrays was based on slides I received from Ruby Gabelrab from Affymetrix.

The whole course, including these two presentations, is (or soon will be) available from our wiki here.

Answer 1

[Ruby Gadelrab]

I would love to help - if you can send me your email address I will get some stuff together for you -I work at affymetrix you can contact me on [e-mail]

[Chris Evelo]

Thanks, that would be super.

Answer 2

[User 59]

Chris this sounds like a lot of stuff that might not exist outside of the information on the Affy site:

• Technical notes
• Tutorials
• White Papers

Whether or not you can repurpose this for teaching is another matter, given the licensing issues.