Question: Annotate Orfs?
1
gravatar for Adrian Pelin
5.8 years ago by
Adrian Pelin2.3k
Canada
Adrian Pelin2.3k wrote:

Hello,

When I get a new assembly and I want a raw annotation, I usually extract ORFs, feed them to blast2go, get the sequence description decided by blast2go and that's it.

What I used to far to extract ORFs is artemis, I like it because it would basically concatenate all contigs into one giant mega contig, and I could tell it not to create ORFs at contig boundaries. That way, the output from blast2go, I could convert it to genbank, and feed it back into artemis.

The problem is that artemis takes longer to perform on bigger genomes.

Any other method you use to annotate your ORFs?

Adrian

orf • 1.7k views
ADD COMMENTlink written 5.8 years ago by Adrian Pelin2.3k
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