I want to use Picard's CalculateHsMetrics tool. To do this one needs a BAIT_INTERVAL & TARGET_INTERVAL file, which both need to contain 5 columns (chromosome, start, end, strand & name). I want to test this on whole exome data. The company has a .bed file with the target (Nextera Exome), but this file does not contain information about the strand. Since this is mandatory for Picard, I was wondering if someone knows how to get this information? Is Picard actually using this information to calculate the metrics?