Hi everyone, I tried to align one of my reference genes with its contigs using Mauve, MAFFT, emboss needle etc, but could not get it aligned locally (I also needed to show the aligned sequences) . Is there any program I can use to do the local pairwise alignment of multiple sequences?? I just need to map the contigs to my reference gene and find the UTR regions and introns. Any inputs would be appreciated on this.. Thanks!!