Question: Microarray And Rna-Seq/Dge
1
gravatar for S.Retina
9.5 years ago by
S.Retina30
S.Retina30 wrote:

What are the advantages and disadvantages of Microarray and RNA-Seq/DGE?

homework rna microarray • 3.4k views
ADD COMMENTlink modified 9.5 years ago by Nibua60 • written 9.5 years ago by S.Retina30
3
gravatar for Steve Lianoglou
9.5 years ago by
Steve Lianoglou5.1k
US
Steve Lianoglou5.1k wrote:

The following (open access(!)) papers should be relevant:

  1. Microarrays, deep sequencing and the true measure of the transcriptome

  2. Protocol Dependence of Sequencing-Based Gene Expression Measurements

ADD COMMENTlink written 9.5 years ago by Steve Lianoglou5.1k
0
gravatar for Nibua
9.5 years ago by
Nibua60
Montpellier, FRANCE
Nibua60 wrote:

Hello,

First, RNA-Seq and DGE are not the same. The protocols differs, and it seems that Illumina has stopped to provide the kit for this technic. RNA-Seq provides a better resolution. Thanks to this you can capture seperatly each transcripts. But you need to be carefull of your coverage. The Mircroarrays are less resolutive and depends of the design of the arrays. And finally the big difference is the cost. And even if you do RNA-Seq you have to make replicates.

ADD COMMENTlink written 9.5 years ago by Nibua60
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