Question: Eqtl Results Adjusted For Genomic Inflation Factor
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gravatar for Zhenyu Zhang
5.5 years ago by
Zhenyu Zhang240
United States
Zhenyu Zhang240 wrote:

I've used MatrixEQTL to find eQTLs and got the p-values. I ran these p-values through GenABEL to get inflation factor lambda. However, what to do next? Based on GWAS analysis, I should divide each Chi-square statistics with this factor, regenerate p-values, and calculate q-values, is it correct? However, for eQTLs, I only have t-stat, should I use t-stat to do the same thing (new_pval = pt ( -abs ( t-stat / lambda ), df ) * 2 ) ? Thanks a lot.

gwas genomic eqtl • 2.2k views
ADD COMMENTlink modified 2.2 years ago by Fabio Marroni2.3k • written 5.5 years ago by Zhenyu Zhang240
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gravatar for Fabio Marroni
2.2 years ago by
Fabio Marroni2.3k
Italy
Fabio Marroni2.3k wrote:

I think that the most correct thing is the following, I hope that someone can confirm (or contradict) this:

1) Transform all your pvalues in chi-square values with one df using qchisq function, and set lower.tail=FALSE

2) Run genabel to estimate lambda (you can estimate lambda based on chisquare values as well)

3) Divide the chisquare values by lambda

4) Compute the pvalues from the new chisquare values using the function pchisq, again setting lower.tail=F

ADD COMMENTlink written 2.2 years ago by Fabio Marroni2.3k
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