Question: Base-Calling Tools Or Softwares For Abi Files Which Has Raw Data Only
gravatar for Kanchana Sankalpa
4.3 years ago by
Sri lanka
Kanchana Sankalpa0 wrote:

We have ABI files which are extracted from Applied Bio-systems 3130 Genetic Analyzer.However the ABI files contain only raw data channel information(DATA 1-4) .These files missing the Base-call information(decrypted with PBAS and PLOC tags)

Is there any free online tool or software available to extent the Base-call information from the raw data channel information available in .ab1 files

ADD COMMENTlink modified 4.3 years ago by madkitty570 • written 4.3 years ago by Kanchana Sankalpa0
gravatar for madkitty
4.3 years ago by
madkitty570 wrote:

You might want to try Geneious Pro and align your ab1 to a reference genome in geneious pro. You can download the reference genome directly from geneious pro and then do a align -> Pairwise alignment. I usually do that from ab1 files only and it shows mutation at a base resolution.

Hope it helps.

ADD COMMENTlink written 4.3 years ago by madkitty570
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