Question: Bacterial Transcriptional Start Site Analysis By Using Rna-Seq Data.
gravatar for liupfskygre
6.6 years ago by
United States
liupfskygre190 wrote:

Dear all,

For the purpose of detecting transcriptional start sites (TSS) in bacteria, is there any special requirement for the library construction?

I have a list of data from strand specific RNA-seq (sorry, I am not quite familiar with the protocol now), are they suitable for TSS analysis?

In order to analyze the transcriptional start site what kinds of tools are available now?


rnaseq bacteria • 2.7k views
ADD COMMENTlink modified 6.6 years ago by Asaf8.4k • written 6.6 years ago by liupfskygre190
gravatar for Asaf
6.6 years ago by
Asaf8.4k wrote:

You should select for 5'ppp by degrading 5'p and 5'OH ends that are cleavage products.

ADD COMMENTlink written 6.6 years ago by Asaf8.4k
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