Bacterial Transcriptional Start Site Analysis By Using Rna-Seq Data.

Question

[liupfskygre]

Dear all,

For the purpose of detecting transcriptional start sites (TSS) in bacteria, is there any special requirement for the library construction?

I have a list of data from strand specific RNA-seq (sorry, I am not quite familiar with the protocol now), are they suitable for TSS analysis?

In order to analyze the transcriptional start site what kinds of tools are available now?

Thanks!

Answer 1

[Asaf]

You should select for 5'ppp by degrading 5'p and 5'OH ends that are cleavage products.