Question: Bacterial Transcriptional Start Site Analysis By Using Rna-Seq Data.
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gravatar for liupfskygre
5.0 years ago by
liupfskygre180
United States
liupfskygre180 wrote:

Dear all,

For the purpose of detecting transcriptional start sites (TSS) in bacteria, is there any special requirement for the library construction?

I have a list of data from strand specific RNA-seq (sorry, I am not quite familiar with the protocol now), are they suitable for TSS analysis?

In order to analyze the transcriptional start site what kinds of tools are available now?

Thanks!

rnaseq bacteria • 2.2k views
ADD COMMENTlink modified 5.0 years ago by Asaf5.2k • written 5.0 years ago by liupfskygre180
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gravatar for Asaf
5.0 years ago by
Asaf5.2k
Israel
Asaf5.2k wrote:

You should select for 5'ppp by degrading 5'p and 5'OH ends that are cleavage products.

ADD COMMENTlink written 5.0 years ago by Asaf5.2k
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