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Question: Sequence Depth Genome
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gravatar for Oduro
9.7 years ago by
Oduro • 0
Oduro • 0 wrote:

how is average sequence depth calculated from number of reads and read length
homework depth-of-coverage sequence • 2.4k views
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modified 9.7 years ago by Boboppie • 550 • written 9.7 years ago by Oduro • 0
1
gravatar for Pasta
9.7 years ago by
Pasta • 1.3k
Switzerland
Pasta • 1.3k wrote:

I understand this concept as equivalent to coverage.

On a genome it means that each base has been sequenced a number of time.

Coverage = (Nb of Reads)*(Read Length) / (Genome Size)
ADD COMMENT • link written 9.7 years ago by Pasta • 1.3k

I think the second line should read:

On a genome it means that each base has on average been sequenced a number of time.

ADD REPLY • link written 9.7 years ago by Chris Evelo ♦ 10k
1
gravatar for Boboppie
9.7 years ago by
Boboppie • 550
Cambridge, UK
Boboppie • 550 wrote:

Relevant questions are:

@Istvan Albert pointed to the "Coverage" section of "Shotgun sequencing" on Wiki, same as @pasta.
ADD COMMENT • link modified 17 months ago by Ram ♦ 32k • written 9.7 years ago by Boboppie • 550
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