Question: Sequence Depth Genome
0
gravatar for Oduro
9.7 years ago by
Oduro0
Oduro0 wrote:

how is average sequence depth calculated from number of reads and read length

ADD COMMENTlink modified 9.7 years ago by Boboppie550 • written 9.7 years ago by Oduro0
1
gravatar for Pasta
9.7 years ago by
Pasta1.3k
Switzerland
Pasta1.3k wrote:

I understand this concept as equivalent to coverage.

On a genome it means that each base has been sequenced a number of time.

Coverage = (Nb of Reads)*(Read Length) / (Genome Size)

ADD COMMENTlink written 9.7 years ago by Pasta1.3k

I think the second line should read:

On a genome it means that each base has on average been sequenced a number of time.

ADD REPLYlink written 9.7 years ago by Chris Evelo10k
1
gravatar for Boboppie
9.7 years ago by
Boboppie550
Cambridge, UK
Boboppie550 wrote:

Relevant questions are:

@Istvan Albert pointed to the "Coverage" section of "Shotgun sequencing" on Wiki, same as @pasta.

ADD COMMENTlink modified 17 months ago by Ram32k • written 9.7 years ago by Boboppie550
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