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• trimming reads from miseq
  Hi I am going to make a reliable draft genome of a cultured bacteria. This is my FastQC report on...
• Doubts regarding phylogeny analysis with protein sequence
  1. While making a phylogenetic tree with a protein sequence is it ok if I include sequences infer...
• How to generate a family level phylogeny?
  Hi all, I want to generate a 16S family level phylogenetic tree of a bacteria family, and includ...
• GO terms depth level
  Is there any tool that allows me to get, given a particular GO term (i.e. GO:0007049) it's level ...
• Hierarchical ranking of gene ontology
  Hi all. I have a question about the gene ontology ranking. For example, GO:0004672 is named as pr...
• G-Blocks for Trimming MSA
  Hello, I want to use G-blocks to trim a Multiple Sequence Alignment. I wanted to know what file ...
• Is It A Good Idea To Trim Rna-Seq Reads Or Just Remove N'S?
  Hi all, I have some RNA-seq dataset of good coverage to analyse. I can see some N's and low qual...
• David enrichment analysis inconsistency
  I have a list of N genes. I used DAVID (http://david.abcc.ncifcrf.gov) in order to get the GO ter...
• Type of trimming
  Hi Colleagues, Regarding 1- Sequence Duplication Levels 2- Kmer Content in RNA Seq data , which...
• Appropriateness of using topology constraints to enforce species tree concordant phylogeny
  In my current project, I am working on inferring a phylogeny for a particular protein. The troubl...
• Go Term Hierarchy -- How To Find All Children Terms From A Parent Term?
  I have a question regarding gene-ontology term relatedness. Say I have a GO term like GO:0044238,...
• 'How To' On Gene Ontology Analysis
  Hi All, I am trying to make a 'HOW TO' on gene ontology analysis. If you know answer of these qu...
• Representing phylogenetic tree at atomic level (C,H,N,O,S) instead of protein sequences
  Hi, To construct phylogenetic tree, we usually consider genes or protein sequences. I have a ide...
• How To Perform A Hierarchical Clustering Using Interactive Heatmaps From Gitools?
  In the latest version of Gitools, Gitools v2.1, we have improved the clustering of heatmaps. Her...