I followed this tutorial to create the object Vis (https://satijalab.org/signac/articles/pbmc_vignette.html). From the object created here, I have been trying the peak calling from signac package for my snATAC data. I keep running into this error. Can someone please tell me how to fix this!
peaks <- CallPeaks( object = vis, group.by = "seurat_clusters", macs2.path = "/usr/local/apps/python-packages/3.7.3/macs2/18.104.22.168/bin/macs2")
Processing file \PROJECTS\outs\fragments.tsv.gz Keeping 2563 cell barcodes Splitting into 13 files Done Processing 102 million lines Error in file(file, "rt") : cannot open the connection In addition: Warning message: In file(file, "rt") : cannot open file 'C:\Users\AppData\Local\Temp\Rtmp04ptdN/0_peaks.narrowPeak': No such file or directory`