I am relatively new to the world of bioinformatics and have some novice questions regarding scRNA-seq data and variant calling. I am wondering if it is ever legitimate to call variants from scRNA sequencing data. Specifically, I would like to know whether de novo variant discovery can be done from this data. I have seen it be said that If so, can this be done in such a way that mitigates PCR bias, and protects from discovering false positive variants?
Thanks for this. It's very helpful