I am working on an experiment where the Genes of interest were not significantly expressed after the DESeq2 analysis. I applied the filter of 0.05 significance p-value and the genes did not appear. The important genes had a p-value of 1. Is there anything else that could be done as a Bioinformatician to proceed with the analysis?
Edit: My experiment design consists of a control and 2 mutation types. I tried to create DESeq2 conditions MA vs WT and MD vs WT. I am getting upregulated and downregulated genes but it does not consist of the genes that I am interested in. The genes that I am interested in had a log fold change value between 0 and -1 and an adjusted p-value of 1