Hello to every one,
I have a question that can we directly run the gatk HaplotypeCaller tool without using the Picard tool to mark the duplicates??? Are they affect on final results for variant calling analysis?
Thanks in advance
GATK Haplotypecaller does not mark duplicates but utlises reads marked as duplicates and ignores them for calling variants. You need to use Picard Markduplicates in order to mark duplicates before variant calling.
This should be helpful: https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels-
Login before adding your answer.
Use of this site constitutes acceptance of our User Agreement and Privacy