sc-ATAC UMIs
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Entering edit mode
10 weeks ago

I'm relatively new to the world of sc-ATAC profiling/sequencing. I am aware that in many RNA seq experiments, including the 10x chromium v2 & v3 UMIs are made use of to enable the correction PCR bias and get better molecular counts from the data. I was wondering, why aren't similar UMI techniques used in sc-ATAC sequencing? At least this isn't the case in the standard "out of the box" 10x sc-ATAC protocol. Presumably there are similar issues that arise from the PCR steps in both methodologies? I am wondering if this doesn't exist because of some fundamental different in the technologies meaning this is not necessary for sc-ATAC or is it more simply that sc-ATAC is less mature than sc-RNA and that in the fullness of time sc-ATAC will eventually become a UMI technology?

Thanks

sc-RNA scATAC NGS UMIs sc-ATAC • 121 views
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